DSpace - Tor Vergata >
Facolt√† di Scienze Matematiche Fisiche e Naturali >
Tesi di dottorato in scienze matematiche e fisiche >

Please use this identifier to cite or link to this item: http://hdl.handle.net/2108/909

Full metadata record

DC FieldValueLanguage
contributor.advisorLoreni, Fabrizio-
contributor.authorIadevaia, Valentina-
date.accessioned2009-05-27T11:15:25Z-
date.available2009-05-27T11:15:25Z-
date.issued2009-05-27T11:15:25Z-
identifier.urihttp://hdl.handle.net/2108/909-
description21. cicloen
description.abstractPIM1 is an oncogenic serine/threonine kinase mainly expressed in hematopoietic cells. Expression of PIM1 is regulated by a variety of growth factors and cytokines from transcriptional to post-translational level. Together with the other two members of the family (PIM2 and PIM3), PIM1 plays a role in hematopoietic cell growth and survival. By studying the molecular mechanisms of Diamond-Blackfan Anemia (DBA) we have found out that PIM1 interacts with the ribosomal protein RPS19 and cosediments with ribosomes. RPS19 gene is mutated in roughly one fourth of DBA patients, causing a defect in hematopoiesis due to an excess of apoptosis of erythroid precursors. To investigate the possible implication of PIM1 in DBA pathogenesis we have here analyzed the expression of this kinase in cultured cell model systems. These include a stably transfected cell line, inducible for PIM1 overexpression (PIM-REx) and an hematopoietic cell line (K562C), inducible for RPS19 downregulation. We have found that the depletion of RPS19 causes a drastic destabilization of PIM1 partly dependent on proteasome. The rapid degradation of PIM1 could be responsible for some defects observed in cells from DBA patients.en
format.extent1427962 bytes-
format.mimetypeapplication/pdf-
language.isoiten
subjectPIM1en
subjectstress ribosomaleen
subjectribosomien
subject.classificationBIO/11 Biologia molecolareen
titleRuolo della chinasi PIM1 in risposta allo stress ribosomaleen
typeDoctoral thesisen
degree.nameDottorato in biologia cellulare e molecolareen
degree.levelDottoratoen
degree.disciplineFacoltà di Scienze Matematiche Fisiche e Naturalien
degree.grantorUniversità degli studi di Roma Tor Vergataen
date.dateofdefenseA.A. 2008/2009en
Appears in Collections:Tesi di dottorato in scienze matematiche e fisiche

Files in This Item:

File Description SizeFormat
Valentina Iadevaia.pdf1394KbAdobe PDFView/Open

Show simple item record

All items in DSpace are protected by copyright, with all rights reserved.