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Please use this identifier to cite or link to this item: http://hdl.handle.net/2108/779

Title: Studio della rete d’interazioni delle proteine di divisione dei procarioti: identificazione del minimum comune divisoma
Authors: Paolozzi, Luciano
D’Ulisse, Valeria
Keywords: proteine di divisione
saggio di doppio ibrido procariotico
coimmunoprecipitazioni
escherichia coli
streptococcus pneumoniae coimmunoprecipitazioni
escherichia coli
streptococcus pneumoniae
Issue Date: 2-Feb-2009
Abstract: FtsQ, an essential protein for the Escherichia coli divisome assembly, is able to interact with various division proteins, namely FtsI, FtsL, FtsN, FtsB and FtsW. In this paper, the FtsQ domains involved in these interactions were identified by two-hybrid assays and co-immunoprecipitations. Progressive deletions of the ftsQ gene suggested that the FtsQ self-interaction and its interactions with the other proteins are localized in three periplasmic subdomains: (i) residues 50-135 constitute one of the sites involved in FtsQ, FtsI and FtsN interaction, and this site is also responsible for FtsW interaction; (ii) the FtsB interaction is localized between residues 136 and 202; and (iii) the FtsL interaction is localized at the very C-terminal extremity. In this third region, the interaction site for FtsK and also the second site for FtsQ, FtsI, FtsN interactions are located. As far as FtsW is concerned, this protein interacts with the fragment of the FtsQ periplasmic domain that spans resi...
Description: 19. ciclo
URI: http://hdl.handle.net/2108/779
Appears in Collections:Tesi di dottorato in scienze matematiche e fisiche

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