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Please use this identifier to cite or link to this item: http://hdl.handle.net/2108/700

Title: Design of human single chain fragments for antibody direct enzyme prodrug therapy (ADEPT)
Authors: Cianfriglia, Maurizio
Zamboni, Silvia
Keywords: scFv
ADEPT
Issue Date: 18-Nov-2008
Abstract: This study describes the genetic construction and expression of a fusion protein consisting of a human monoclonal antibody in a single chain fragment variable (scFv) format (E8) specific for a well defined determinant of the CEA cell surface antigen family and cytosine deaminase from yeast (yCD). DNA sequence encoding for the scFvE8 human monoclonal antibody recognizing an epitope shared by CEACAM1 (CD66) and CEACAM5 isoforms was assembled with a monomer of yCD. The scFvE8:yCD fusion protein possessed the binding specificity of the immuncompetent part of protein which include the recognition of melanoma (Mel P5) and colon carcinoma (LoVo) cell lines. The scFv8:yCD system showed the ability to make tumour cells naturally resistant to chemotherapy, susceptible to the non toxic substrate 5-fluorocytosine (5-FC) by its enzymatic conversion into 5-fluorouracil (5-FU). In vitro pre-treatment of Mel P5 and LoVo cell lines with scFvE8:yCD followed by cell washing and incubation with 5-FC, res...
Description: 20. ciclo
URI: http://hdl.handle.net/2108/700
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