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Please use this identifier to cite or link to this item: http://hdl.handle.net/2108/586

Title: Potential role of IL-10-treated dendritic cells in the control of the immune response to allergens
Authors: Angelini, Federica
Pacciani, Valentina
Keywords: allergy
dendritic cells
regulatory T cells
Issue Date: 28-Aug-2008
Abstract: Several lines of evidence indicate that a defect in immunoregulatory mechanisms is involved in the pathogenesis of allergic asthma. The aim of this study is to determine whether IL-10-treated dendritic cells (DC) are able to modulate allergen-specific T cell responses in children affected by allergic asthma. 41 children (4-14 years) allergic to House Dust Mite (HDM), and 10 healthy age-matched children were recruited. DC were differentiated from peripheral blood CD14+ precursors and cultured with GM-CSF and IL-4 for 5 days. Der p2 (a major HDM allergen) was added alone or in combination with IL-10 for 48 hours to obtain Dp2-DC and IL10 Dp2-DC, respectively. Alternatively, DC were differentiated in the presence of IL-10 and pulsed with Der p2 during the 2 last days of culture (Dp2-DC10). The ability of the resulting DC to stimulate allergen-specific autologous T cells and to promote allergen-specific T cell anergy was analyzed. Dp2-DC induced allergen-specific T cell proliferati...
Description: 19. ciclo
URI: http://hdl.handle.net/2108/586
Appears in Collections:Tesi di dottorato in medicina

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