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|Title: ||Potential role of IL-10-treated dendritic cells in the control of the immune response to allergens|
|Authors: ||Angelini, Federica|
regulatory T cells
|Issue Date: ||28-Aug-2008 |
|Abstract: ||Several lines of evidence indicate that a defect in immunoregulatory mechanisms is involved in the pathogenesis of allergic asthma.
The aim of this study is to determine whether IL-10-treated dendritic cells (DC) are able to modulate allergen-specific T cell responses in children affected by allergic asthma.
41 children (4-14 years) allergic to House Dust Mite (HDM), and 10 healthy age-matched children were recruited. DC were differentiated from peripheral blood CD14+ precursors and cultured with GM-CSF and IL-4 for 5 days. Der p2 (a major HDM allergen) was added alone or in combination with IL-10 for 48 hours to obtain Dp2-DC and IL10 Dp2-DC, respectively. Alternatively, DC were differentiated in the presence of IL-10 and pulsed with Der p2 during the 2 last days of culture (Dp2-DC10).
The ability of the resulting DC to stimulate allergen-specific autologous T cells and to promote allergen-specific T cell anergy was analyzed.
Dp2-DC induced allergen-specific T cell proliferati...|
|Description: ||19. ciclo|
|Appears in Collections:||Tesi di dottorato in medicina|
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