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Please use this identifier to cite or link to this item: http://hdl.handle.net/2108/548

Title: Biologia molecolare della paraoxonasi
Authors: Cortese, Claudio
Liberatoscioli, Laura
Keywords: paraoxonase
high density lipoproteins
genetic polymorphisms
allelic discrimination
Issue Date: 9-Jul-2008
Abstract: Human serum paraoxonase (PON1) is an HDL-associated enzyme involved in the protection of lipoproteins from oxidation. Endothelial dysfunction has been documented in subjects with diabetes mellitus and is probably related to increased oxidation of circulating LDL. A genetic polymorphism of the PON1 (Gln192Arg) has been suggested to influence its anti-oxidant capacity, with the Arg allele associated with lesser protection of LDL against the accumulation of lipid peroxides. The first step of the present study was to test a fluorescent 5’ exonuclease assay to detect the point mutation in the paraoxonase gene (Q192R). This assay takes advantage of 5’ exonuclease activity of Taq DNA polymerase to cause the cleavage of two allelic-specific probes, which hybridized to template DNA during the PCR and that determine an increase of reporter fluorescence in solutions. This assay was applied to 120 sample of genomic DNA, extracted from whole blood, and we had a perfect allelic discriminations betwe...
Description: 19. ciclo
URI: http://hdl.handle.net/2108/548
Appears in Collections:Tesi di dottorato in scienze matematiche e fisiche

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