DSpace - Tor Vergata: Item 2108/482

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Title:	Identification of novel and direct target genes of p73
Authors:	Blandino, Giovanni Lapi, Eleonora
Keywords:	p73 p63 YAP S100A2 PML differentiation apoptosis
Issue Date:	13-May-2008
Abstract:	The p53 paralogues p73, p63 and their respective truncated isoforms have been shown to be critical regulators of developmental and differentiation processes. Indeed, both p73 and p63 deficient mice exhibit severe developmental defects. Here, we show that the S100A2 gene, whose transcript and protein are induced during keratinocyte differentiation of HaCaT cells, is a direct transcriptional target of p73β and ΔNp63α and is required for proper keratinocyte differentiation. Transactivation assays reveal that p73β and ΔNp63α exert opposite transcriptional effects on the S100A2 gene. While ΔNp63α is found in vivo onto S100A2 regulatory regions predominantly in proliferating cells, p73β is recruited in differentiating cells. Silencing of p73 impairs the induction of S100A2 during the differentiation of HaCaT cells. Moreover, silencing of p73 or S100A2 impairs the proper expression of keratinocyte differentiation markers. Of note, p53 family members do not trigger S100A2 gene ...
Description:	19. ciclo
URI:	http://hdl.handle.net/2108/482
Appears in Collections:	Tesi di dottorato in scienze matematiche e fisiche

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